A common and inexpensive protein, egg albumin, was applied to the solid-liquid separation or flocculation of diatomite. Egg albumin was methylated in a 0.1 M HCl methyl alcohol solution at room temperature. About 90% of the carboxylic groups of egg albumin could be methylated within 24 h. The adsorption of egg albumin to diatomite at pH6.8 was remarkably enhanced by methylation. The adsorption constant of methylated egg albumin to diatomite at 30°C was about 100-fold larger than that of native egg albumin however the adsorption constant of methylated egg albumin decreased to about one-hundredth with decreasing temperature from 30 to 6°C. The saturated adsorption amount of egg albumin was also increased by the methylation. The flocculating ability of methylated egg albumin was examined with a diatomite suspension at 6 and 30°C in the pH range from pH2 to pH11. The diatomite suspension was effectively flocculated by the addition of small amount of methylated egg albumin (only 0.5-1 wt-% against diatomite) over a wide pH range from pH 3 to pH10.
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机译：将一种常见且廉价的蛋白质蛋清应用于硅藻土的固液分离或絮凝。在室温下，将蛋清蛋白在0.1 M HCl甲醇溶液中甲基化。鸡蛋清蛋白中约90％的羧基可在24小时内甲基化。甲基化显着增强了鸡蛋白蛋白在pH6.8时对硅藻土的吸附。甲基化蛋清的吸附常数在30°C时比天然蛋清的吸附常数大100倍，但是随着温度从30降低到6°C，甲基化蛋清的吸附常数降低到大约一百分之一。蛋清的饱和吸附量也通过甲基化而增加。用硅藻土悬浮液在6和30℃，pH2到pH11的范围内检查了甲基化蛋清的絮凝能力。通过在pH 3至pH10的宽pH范围内添加少量的甲基化蛋清蛋白（相对于硅藻土仅0.5-1 wt％），可以有效地絮凝硅藻土悬浮液。
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